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lb agar plates  (Thermo Fisher)


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    Thermo Fisher lb agar plates
    Lb Agar Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/agar+plates/Kanamycin+monosulfate/pm42176792-240-31-39
    Average 94 stars, based on 1 article reviews
    lb agar plates - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Suspension:

    Article Title: Antimicrobial resistance and phylogenetic diversity of Escherichia coli isolates from coastal recreational waters in Costa Rica.
    Article Snippet: Few studies have examined the antimicrobial resistance profiles and phylogenetic diversity of bacteria in Latin American beaches.. The aim of this study is to provide nationwide data on Escherichia coli from recreational beaches along the Costa Rican coasts, contributing to the understanding of this knowledge gap.. Thirty-nine strains were recovered, one per sampled site, and tested for susceptibility to eleven antibiotics using the disk diffusion method.

    Transformation Assay:

    Article Title: The C-terminus of infectious bursal disease virus VP3 encodes a predicted intrinsically disordered region, which promotes the formation of cytoplasmic puncta and modulates their physical properties
    Article Snippet: The ligation product was then used to transform chemically competent E. coli (NEB), according to the manufacturer’s protocols. .. After transformation and outgrowth in SOC media (ThermoFisher Scientific), the mixture was streaked onto agar plates with the appropriate antibiotic selection marker (ThermoFisher Scientific). ..

    Selection:

    Article Title: The C-terminus of infectious bursal disease virus VP3 encodes a predicted intrinsically disordered region, which promotes the formation of cytoplasmic puncta and modulates their physical properties
    Article Snippet: The ligation product was then used to transform chemically competent E. coli (NEB), according to the manufacturer’s protocols. .. After transformation and outgrowth in SOC media (ThermoFisher Scientific), the mixture was streaked onto agar plates with the appropriate antibiotic selection marker (ThermoFisher Scientific). ..

    Marker:

    Article Title: The C-terminus of infectious bursal disease virus VP3 encodes a predicted intrinsically disordered region, which promotes the formation of cytoplasmic puncta and modulates their physical properties
    Article Snippet: The ligation product was then used to transform chemically competent E. coli (NEB), according to the manufacturer’s protocols. .. After transformation and outgrowth in SOC media (ThermoFisher Scientific), the mixture was streaked onto agar plates with the appropriate antibiotic selection marker (ThermoFisher Scientific). ..

    Activity Assay:

    Article Title: Characterization of Infant Formulae Marketed in Italy and Virulence Potential of Bacillus cereus Isolates
    Article Snippet: Proteolytic activity was tested by growing the isolates on 1.2% agar plates containing 1.5% skim milk (Oxoid, UK) at 37 °C for 18 h [ ]; protease production was indicated by clear zones surrounding the colonies. .. Proteolytic activity was tested by growing the isolates on 1.2% agar plates containing 1.5% skim milk (Oxoid, UK) at 37 °C for 18 h [ ]; protease production was indicated by clear zones surrounding the colonies. ..

    Article Title: Characterization of Infant Formulae Marketed in Italy and Virulence Potential of Bacillus cereus Isolates
    Article Snippet: Proteolytic activity was tested by growing the isolates on 1.2% agar plates containing 1.5% skim milk (Oxoid, UK) at 37 ◦C for 18 h [47]; protease production was indicated by clear zones surrounding the colonies. .. Proteolytic activity was tested by growing the isolates on 1.2% agar plates containing 1.5% skim milk (Oxoid, UK) at 37 ◦C for 18 h [47]; protease production was indicated by clear zones surrounding the colonies. ..

    Article Title: A novel polyhydroxyalkanoate-storing bacterium Thauera carbonocopians sp. nov. isolated from a sequencing batch reactor fed with volatile fatty acids
    Article Snippet: .. Agar plates were placed in an anaerobic chamber (Oxoid, UK), and inoculated tubes containing 5 mL of medium were overlaid with 2 mL of sterilized mineral oil and incubated at 27 °C for 5 days. (v) Nitrate reduction was tested in TSB supplemented with 0.1% KNO 3 by detecting nitrite with naphthylamine/sulfanilic acid reagents and residual nitrate with zinc powder . (vi) Oxidase activity was determined by streaking biomass on filter paper soaked with 1% (w/v) N,N,N′,N′-tetramethyl-p-phenylenediamine and observing a colour change to purple . (vii) Catalase activity was detected by adding 3% ( v/v ) H 2 O 2 solution to the biomass and observing bubble formation. (viii) Motility was assessed on semi-solid medium containing 0.4% ( w/v ) agar . .. Utilization of different substrates was tested using Biolog Phenotypic MicroArray plates PM1 and PM2A (Biolog, USA) with modified minimal Brunner medium .

    Incubation:

    Article Title: A novel polyhydroxyalkanoate-storing bacterium Thauera carbonocopians sp. nov. isolated from a sequencing batch reactor fed with volatile fatty acids
    Article Snippet: .. Agar plates were placed in an anaerobic chamber (Oxoid, UK), and inoculated tubes containing 5 mL of medium were overlaid with 2 mL of sterilized mineral oil and incubated at 27 °C for 5 days. (v) Nitrate reduction was tested in TSB supplemented with 0.1% KNO 3 by detecting nitrite with naphthylamine/sulfanilic acid reagents and residual nitrate with zinc powder . (vi) Oxidase activity was determined by streaking biomass on filter paper soaked with 1% (w/v) N,N,N′,N′-tetramethyl-p-phenylenediamine and observing a colour change to purple . (vii) Catalase activity was detected by adding 3% ( v/v ) H 2 O 2 solution to the biomass and observing bubble formation. (viii) Motility was assessed on semi-solid medium containing 0.4% ( w/v ) agar . .. Utilization of different substrates was tested using Biolog Phenotypic MicroArray plates PM1 and PM2A (Biolog, USA) with modified minimal Brunner medium .

    Saline:

    Article Title: Phosphorothioate DNA modification by BREX type 4 systems in the human gut microbiome
    Article Snippet: Parabacteroides spp. and Bacteroides spp., E. faecalis , and BIO-ML isolates were grown on ASF plates (Becton Dickinson) and BHIS plates , BHI plates (Becton Dickinson), and Brucella agar (Catalog #AS-141, Anaerobe Systems, Morgan Hill, CA), respectively. .. Growth on agar plates was harvested with phosphate-buffered saline (Catalog #10010-023, Gibco, Paisley, PA). .. Growth on agar plates was harvested with phosphate-buffered saline (Catalog #10010-023, Gibco, Paisley, PA).

    Article Title: Phosphorothioate DNA modification by BREX type 4 systems in the human gut microbiome.
    Article Snippet: .. Growth on agar plates was harvested with phosphate buffered saline (Catalog # 10010-023, Gibco, Paisley, PA). ..



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    Isolation, characterization, and phenotypic analysis of HCC tumor-resident S. epidermidis (A) Summary of viable bacterial species isolated from the T and PT tissues of four HCC patients. Species identification was confirmed by 16S/18S rRNA sequencing and MALDI-TOF mass spectrometry. (B) FISH images showing the spatial localization of S. epidermidis within HCC tumor tissue. Green signals correspond to the S. epidermidis- specific or E. cloacae -specific probe, red signals correspond to the K. pneumoniae- specific probe, while blue signals represent the cell nuclei stained with DAPI. Scale bars, 20 μm. (C) Comparison of detection methods for S. epidermidis across the four patient samples. Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on <t>Columbia</t> <t>blood</t> <t>agar</t> <t>plates</t> after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains. The arrows highlight the raised granular structures on the surface of the clinical isolates. Scale bars, 500 nm. (F) Growth curves of the GX-1, GX3-2, and ATCC 12228 strains cultured in BHI medium. The optical density (OD) was measured at 600 nm over 24 h. (G) Representative growth inhibition curves for S. epidermidis GX3-2. Bacterial growth, measured by OD 600 , is plotted against serially diluted concentrations of various antibiotics. The data are presented as the mean ± SEM from three independent experiments.
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    Image Search Results


    Isolation, characterization, and phenotypic analysis of HCC tumor-resident S. epidermidis (A) Summary of viable bacterial species isolated from the T and PT tissues of four HCC patients. Species identification was confirmed by 16S/18S rRNA sequencing and MALDI-TOF mass spectrometry. (B) FISH images showing the spatial localization of S. epidermidis within HCC tumor tissue. Green signals correspond to the S. epidermidis- specific or E. cloacae -specific probe, red signals correspond to the K. pneumoniae- specific probe, while blue signals represent the cell nuclei stained with DAPI. Scale bars, 20 μm. (C) Comparison of detection methods for S. epidermidis across the four patient samples. Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on Columbia blood agar plates after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains. The arrows highlight the raised granular structures on the surface of the clinical isolates. Scale bars, 500 nm. (F) Growth curves of the GX-1, GX3-2, and ATCC 12228 strains cultured in BHI medium. The optical density (OD) was measured at 600 nm over 24 h. (G) Representative growth inhibition curves for S. epidermidis GX3-2. Bacterial growth, measured by OD 600 , is plotted against serially diluted concentrations of various antibiotics. The data are presented as the mean ± SEM from three independent experiments.

    Journal: iScience

    Article Title: A tumor-resident Staphylococcus epidermidis strain exhibits genomic and metabolic traits driving hepatocellular carcinoma progression

    doi: 10.1016/j.isci.2026.116084

    Figure Lengend Snippet: Isolation, characterization, and phenotypic analysis of HCC tumor-resident S. epidermidis (A) Summary of viable bacterial species isolated from the T and PT tissues of four HCC patients. Species identification was confirmed by 16S/18S rRNA sequencing and MALDI-TOF mass spectrometry. (B) FISH images showing the spatial localization of S. epidermidis within HCC tumor tissue. Green signals correspond to the S. epidermidis- specific or E. cloacae -specific probe, red signals correspond to the K. pneumoniae- specific probe, while blue signals represent the cell nuclei stained with DAPI. Scale bars, 20 μm. (C) Comparison of detection methods for S. epidermidis across the four patient samples. Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on Columbia blood agar plates after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains. The arrows highlight the raised granular structures on the surface of the clinical isolates. Scale bars, 500 nm. (F) Growth curves of the GX-1, GX3-2, and ATCC 12228 strains cultured in BHI medium. The optical density (OD) was measured at 600 nm over 24 h. (G) Representative growth inhibition curves for S. epidermidis GX3-2. Bacterial growth, measured by OD 600 , is plotted against serially diluted concentrations of various antibiotics. The data are presented as the mean ± SEM from three independent experiments.

    Article Snippet: Positive detection by either culture-based isolation or FISH is indicated. (D) Colony morphologies of GX-1 and GX3-2 compared with those of the reference strain ATCC 12228 on Columbia blood agar plates after 24 h of incubation. (E) SEM images revealing the ultrastructures of GX-1, GX3-2, and ATCC 12228 strains.

    Techniques: Isolation, Sequencing, Mass Spectrometry, Staining, Comparison, Incubation, Cell Culture, Inhibition